Archaeome in Colorectal Cancer: High Abundance of Methanogenic Archaea in Colorectal Cancer Patients

Background: The importance of microbiome in the progression and development colorectal cancer (CRC) has been discussed last decade. Like colon bacteria, other intestinal microorganisms, including archaea, could also be involved CRC progression, so it's important to work out archaeal (archaeome) composition among patients. Objectives: aim this study was determine archaeome healthy controls based on age gender. Methods: Total bacterial DNA extracted from 30 biopsy samples (17 13 controls). Archaeome communities were profiled by 16S rRNA high throughput sequencing, then compared clinicopathological features, patients’ gender age. Results: In patients, methanogens Methanobrevibacter (86%) Methanomassiliicoccus (8%) overrepresented at genus level. contrast controls, only two genera haloarchaea Natronococcus (58%) Haloterrigena (42%) presented. results showed that number men is higher than women both controls. moreover, our most archaea are present CRC-32-50 group, six genera. differential abundance taxa analysis significant differences between patients (P ≤ 0.05). Conclusions: suggests may development.